Residue-specific immobilization of protein molecules by size-selected clusters
نویسندگان
چکیده
منابع مشابه
Size Selected Clusters on Surfaces
Size selected metal oxide, sulfide and oxynitride clusters, soft-landed onto highly ordered pyrolytic graphite (HOPG) at room temperature, have been studied in this work. Based on their size and chemical compositions, the deposited clusters have exhibited various surface structures as illustrated by in situ Scanning Tunnelling Microscope (STM) and ex situ Atomic Force Microscope (AFM). In contr...
متن کاملSite-specific protein immobilization by Staudinger ligation.
The Staudinger ligation between an azido-protein and a phosphinothioester-derivatized surface is demonstrated to be an effective means for the site-specific, covalent immobilization of a protein. Immobilization yields of >50% are obtained in <1 min, and immobilized proteins have >80% of their expected activity. No other method enables more rapid immobilization or a higher yield of active protei...
متن کاملElectron capture by finite-size polarizable molecules and clusters.
The effects of finite molecular target size are investigated for partial-wave selected capture of electrons by isotropically polarizable molecules and clusters within a generalized Vogt-Wannier model. It is shown how expressions for partial-wave selected capture probabilities of zero-size targets from Dashevskaya et al. (Phys. Chem. Chem. Phys., 2009, 11, 9364) can be modified to account for fi...
متن کاملGeneral method for site-specific protein immobilization by Staudinger ligation.
Protein microarrays are playing an increasingly important role in the discovery and characterization of protein-ligand interactions. The uniform orientation conferred by site-specific immobilization is a demonstrable advantage in using such microarrays. Here, we report on a general strategy for fabricating gold surfaces displaying a protein in a uniform orientation. An azido group was installed...
متن کاملSpectroscopy and reactivity of size-selected Mg+ -ammonia clusters.
Photodissociation spectra for mass-selected Mg(+)(NH(3))(n) clusters for n=1 to 7 are reported over the photon energy range from 7000 to 38 500 cm(-1). The singly solvated cluster, which dissociates primarily via a N-H bond cleavage, exhibits a resolved vibrational structure corresponding to two progressions in the intracluster Mg(+)-NH(3) modes. The addition of the second, third, and fourth so...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of The Royal Society Interface
سال: 2005
ISSN: 1742-5689,1742-5662
DOI: 10.1098/rsif.2005.0032